Slicing parameters

Indicates whether a sequence should be sliced or not. Turn off this option when sequence/input file already contains sequence fragments.
Nucleic acid slicing length.
How many times will simulate sequence fragmentation

Simulation parameters

The following parameters control the hydridization simulation done by UNAFold. If multiple values are given for one or more parameters, UNAFold will be run for every combination.


Sets the nucleic acid type to DNA or RNA
Temperature of the simulation. Multiple values can be given, e.g., 37,39,41,43,45 or 37-45:2
Sodium concentration in M. Multiple values can be given, e.g., 1,1.5,2,2.5,3 or 1-3:0.5
Magnesium concentration in M. Multiple values can be given, e.g., 1,1.5,2,2.5,3 or 1-3:0.5